Overview
openUC2 Light-sheet Microscope
Autofocussing, XYZ Sample scanning, Single-mode laser, water sample chamber,...
Modules
Enhance the functionality of your Scope
Community-driven Software
ImSwitch
Modular Microscopy Software
Open-Source
The adaptable System for your modular Idea
The resolution of any microscope is limited not only in the lateral direction but also in the axial direction. Light-sheet microscopy significantly enhances optical sectioning of a sample by using an illumination sheet perpendicular to the detection axis, selectively exciting the fluorescent sample. By moving the sample through the static light sheet and simultaneously capturing a stack of images, a 3D volume can be quickly generated. This method not only improves resolution along the optical axis but also offers the advantage of significantly reduced photodamage compared to confocal microscopy.
The openSPIM design has been around for years, often with a six-figure price tag, making it inaccessible to many. The openUC2 light-sheet benefits from the modular, cube-based arrangement of optical components, enabling rapid prototyping. This is especially advantageous for educational purposes, where one can learn the setup and alignment without the risk of damaging expensive components. Furthermore, this entry-level device is excellent for making custom hardware modifications by swapping modules or quickly testing new biological protocols (e.g., clearing). Intrigued? Reach out to us!
Light-Sheet Microscopy
Features
Objectives:
- 4x, NA=0.15, WD: 20mm, (FOV: 10mm, Res.: 2.3μm)
- 10x, NA=0.3 WD: 14mm, (FOV: 4mm, Res.: 1.2μm)
Illumination (Configurable):–
- Transmission Brightfield LED
- 488nm Single-Mode, ca. 40 mW (Fluorescence)
- 635nm Single-Mode ca. 200 mW (Fluorescence)
- 488nm/635nm Single Mode Dual Colour Laser (Fluorescence)
Camera:
- 12 MP, USB 3.0, Sony IMX226
- 6 MP, USB 3.0, Sony IMX179
Objective Focusing Stage:
- +/-25mm, 0.3nm increment open-loop
- fully metall-based
Sample Stage:
- +/-10mm, 0.3nm increment open-loop
- fully metall-based
Sample Chamber:
- 3D printed, Volume ca. 10 x 10 x 10 mm^3
- Two windows for excitation and emission
- Inbuilt LED for transmission excitation
- Magnetic quick-mounting mechanism
openUC2e:
- ESP32-based
- Laser, LED control
- Stepper control
- Powered by UC2-ESP firmware
Customizable
Adapt to your sample
Discover the latest innovation in microscopy with our new XYZ stage, designed to enhance flexibility for both your application and samples. Effortlessly accommodate standard well-plates, or utilize our customized sample adaptors for seamless integration of any sample holder.
Our microscope sets itself apart by keeping the optics fixed, allowing the sample to move within a stationary focus, offering a unique approach compared to traditional designs. This open-source architecture not only facilitates easy customization but also simplifies the incorporation of additional components like cell incubation chambers.
The XY stage is powered by fastly moving stepper motors, achieving a resolution of ~0.3 µm, complemented by a magnetic encoder that ensures a control feedback loop as precise as 1µm. Moreover, the Z-axis benefits from a dual sub-micrometer precise linear translational stage mechanism, enabling smooth movement of the sample plate. Embrace unparalleled precision and adaptability with our innovative XYZ stage microscope, designed for advanced research needs.
Modular
Customize your scope
All interfaces of the device are fully open and can be controlled by the open-source microscopy control, acquisition, and processing software “ImSwitch.” This provides full control over all hardware and imaging components. Custom algorithms such as tracking, deconvolution, etc., can be integrated. The software features a Quickscan function that quickly captures and renders the volume during movement. Two different industry-grade CMOS cameras are available: one is compatible with both MAC and Windows, making it excellent for educational purposes, while the other, with significantly better noise statistics, is aimed primarily at research applications.
Portable
Field-trip ready.
Bringing samples from field research back to your lab often presents immense challenges. Limited space or sample degradation can hinder the study of specimens, making on-site research highly advantageous. With the openUC2 system, this is no longer a problem. The robust case allows for transport to remote locations and even passes through airport security checks. Weighing just 3KG, it is not much heavier than a laptop. Powered by a 12V battery connected to your laptop, it offers maximum mobility and enables you to conduct experiments far from any power outlet.
You want better Sectioning for Your biological Tissue?
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